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KMID : 0380020100250030239
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Korean Journal of Biotechnology and Bioengineering
2010 Volume.25 No. 3 p.239 ~ p.243
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Characteristics of Polyhydroxyalkanoates Synthesis by Ralstonia eutropha from Vegetable Oils
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Park Dae-Hoo
Kim Beom-Soo
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Abstract
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Six strains of Ralstonia eutropha were grown to investigate characteristics of polyhydroxyalkanoates (PHA) synthesis from vegetable oils or glycerol. Poly(3-hydroxybutyrate) homopolymer was formed using soybean oil, olive oil, or glycerol as carbon source, while poly(3-hydroxybutyrate-co-4-hydroxybutyrate) or poly(3-hydroxybutyrateco- 3-hydroxyvalerate) copolymers were synthesized by co-feeding γ-butyrolactone or pentanoic acid, respectively. Optimum strain was determined as R. eutropha KCTC 2662 in terms of final cell concentration and PHA content. From 20 g/L of soybean oil (optimum substrate), cell concentration and PHA content at 72 h ranged 1.7~9.2 g/L and 70~92 wt%, respectively.
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KEYWORD
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polyhydroxyalkanoates, PHA, Ralstonia eutropha, vegetable oil, glycerol
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